Translational_Unit

Part:BBa_K515002:Design

Designed by: Atipat Patharagulpong   Group: iGEM11_Imperial_College_London   (2011-09-01)

PA2652


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 675
    Illegal NgoMIV site found at 777
    Illegal AgeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 984


Design Notes

- Sequence is codon optimised for E. coli & B. subtilis.
- Insulator allows interchangeability of promoters without influencing the strength of the RBS.
- To extract this part from biobrick BBa_K515102 use PCR with insulator as starting sequence for primers.


Source

Protein product is originally from Pseudomonas aeruginosa strain PA01 . DNA sequence was codon optimised using our codon optimisation software and synthesised by Eurofins. Insulator was developed separately. RBS was generated using Salis Lab RBS calculator v1.1.

References